Tr-fret fxr coactivator recruitment assay
WebThe LanthaScreen TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as … WebThe most common lanthanides used in TR-FRET assays for HTS are terbium and europium. Terbium offers unique advantages over europium when used as the donor species in a TR-FRET assay. In contrast to europium-based systems that employ APC as the acceptor, terbium-based TR-FRET assays can use common fluorophores such as fluorescein as the …
Tr-fret fxr coactivator recruitment assay
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WebThe results are in agreement with the results from the TR-FRET coactivator recruitment assays, where MRL-871 and Bit-L15 show a similar overall affinity for RORγt, while MRL-L15 is less potent ... WebThe selected compounds are then tested biochemically for their ability to modulate FXR-coactivator binding interactions using a time-resolved fluorescence resonance energy …
WebFigure 2—AR TR-FRET coactivator recruitment assay The TR-FRET ratio was measured after 1 hour for reactions containing 20 nM AR-LBD (GST, 1 µM fluorescein-labeled … WebCatalog number: PV4833. The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput …
WebInvitrogen • LanthaScreen™ TR-FRET Liver X Receptor beta Coactivator Assay Page 3 of 11 3. INTRODUCTION Invitrogen’s LanthaScreen™ TR-FRET Liver X Receptor beta … WebSep 26, 2014 · The FXR-bla assay is able to ... The antagonist assay was conducted in the presence of 50 μM CDCA to induce coactivator recruitment. The TR-FRET ratios were normalized using DMSO as 0% ...
Webtaken to study the differential recruitment of coactivator and corepressor peptides by tr-Fret for peroxisome proliferator receptors20 and for the thyroid receptor.21 In these reports, …
WebThe LanthaScreen® TR-FRET PR Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential PR ligands as agonists of coactivator recruitment. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a PR ligand-binding domain that is tagged with glutathione-S-transferase hamster bar chewingWebThe LanthaScreen® TR-FRET GR Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential GR ligands as agonists of coactivator … bury domestic abuse serviceWebFigure 2—AR TR-FRET coactivator recruitment assay The TR-FRET ratio was measured after 1 hour for reactions containing 20 nM AR-LBD (GST, 1 µM fluorescein-labeled coactivator peptide (Table 1), 5 µM ligand or equivalent concentration of solvent (shown in key), and 5 nM Terbium anti-GST antibody. The three most reactive peptides are shown. bury dog fenceWebNov 3, 2024 · This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4833: The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) … bury down hillfortWebThe coactivator recruitment assay for FXR activation was carried out with the three variants of the FXR protein by using ... a saturated hyperbolic fluorescence signal curve was … hamster bathing powderWebThe LanthaScreen® TR-FRET Peroxisome Proliferator Activated Receptor gamma Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential PPAR gamma ligands as agonists of coactivator recruitment. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a PPAR bury down didcotWebGUDCA and TUDCA are identified FXR antagonists a, TR-FRET FXR coactivator recruitment assay to assess whether GUDCA is a FXR antagonist in the presence of CDCA (20 μM). n … hamster bathing