Facs antibody ca125 apc
WebCells were harvested, stained with FITC-mouse anti human CD14 antibody (Cat. No. 555397), fixed, permeabilized, and then stained with 0.125 µg of APC-C11.5 antibody (Cat. No. 554576), following Pharmingen's staining protocol (far left panel). The data reflect gating on monocytes, based on forward and side scattered light signals. WebThe CD25 antibody, clone 2A3. is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with PHA-activated human …
Facs antibody ca125 apc
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WebAntibody Type Monoclonal Host Species Mouse Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) Preparation The antibody … WebAug 9, 2024 · 1. Label flow cytometry tubes with CD4 + T cell panel, keep one tube labeled as cells alone. 2. Aliquot 200 μL of cell suspension into labeled FACS tubes (approximately one million cells per tube). 3. Set aside the cells alone tube (unstained by antibodies) to allow for correct setup of voltages on the flow cytometer.
WebAnti-CA125 antibody [X75] Applications: WB, ELISA, ELISA-S, FCM Reactivity: Human (Hu) Conjugate/Tag: Unconjugated Quantity: 100 µg Citations: (2) Supplier Page Sign In … WebFACS: Abbreviation for fluorescence-activated cell sorter .
WebCommonly used tandem fluorochromes used for flow cytometry such as PerCP-Cy5.5, PerCP-eFluor710, PE-Cy7, APC-Cy7, BV605, BV650, BV711, BV786, etc. vary in their ability to transfer energy from donor dye to acceptor dye across antibody lots and over time due to fluorescence resonance energy transfer differences. WebFluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It sorts a heterogeneous mixture of biological cells into two or more containers, one cell at a time, …
WebGeneral notes. This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility. - Improved sensitivity and specificity. - Long-term security …
WebKeep the fluorochrome-conjugated anti-BrdU antibody and 7-AAD protected from light. Unused portions of 1X BD Cytofix/Cytoperm Buffer can be stored at 2 to 8°C. BrdU and DNase. Store the BrdU and DNase at –80°C. The BrdU solution has been shown to be stable for up to 4 months at 2 to 8°C, or it can be refrozen. selma family practiceWebOvarian Cancer Antigen CA125 (CA125/MUC16) was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using … selma fire dept hiringWebCD3 Antibodies. The CD3 antigen is a complex of 5 invariable polypeptidic chains: γ, δ, ε, ζ and η, whose molecular weights are respectively 25-28, 21, 20, 16 and 22 kDa. The CD3 chains are clustered in a group of two invariant dimers, γ/ε and δ/ε associated with a variable dimer which consists of ζ homodimers, or ζ /η, or ζ/γ FcR ... selma fireworksWebHeNe 633 Red APC Alexa Fluor® 647 Infrared BD APC-H7 APC-Cy7 *†BD FACSCanto™ II flow cytometry system Solid State 488 Green FITC Alexa Fluor® 488 Yellow PE Orange PE-Texas Red®b Red PerCP PerCP-Cy5.5 Infrared PE-Cy7 HeNe 633 Red APC Alexa Fluor® 647 Far Red Alexa Fluor® 700b Infrared BD APC-H7 APC-Cy7 Solid Stateb 405 … selma food for lessWebAnti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31. 8 Additional reported applications (for the relevant formats) include: immunoprecipitation 1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions 1-4, and immunohistochemical staining 5,6,7 of acetone-fixed frozen sections ... selma flower shop ncWebAPC-Cy7 is a tandem fluorochrome composed of Allophycocyanin (APC), which is excited by laser lines between 595 and 647 nm and serves as an energy donor, coupled to the cyanine dye Cy7™, which acts as an energy acceptor and fluoresces at 780 nm. BD Biosciences Pharmingen has maximized the fluorochrome energy transfer in APC-Cy7, … selma food bank applicationWebELISA Detection1-3 or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 & 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard. selma food pantry